A ratiometric fluorescence sensor for ascorbic acid determination based on an AND-NAND logic pair

An AND-NAND logic pair is reported based on non-purified carbon quantum dots (CDs) for ascorbic acid (AA) detection. In the logic operation, molybdenum oxide nanosheets (MoO₃ NSs) and AA are used as two signal inputs. In the presence of AA, MoO₃ NSs are reduced to plasmonic molybdenum oxide, which decreases the CD fluorescence intensity because of a static quenching, dynamic quenching, and internal filtration effect. Meanwhile, the AA is oxidized to dehydroascorbic acid and forms fluorescent 3-(dihydroxyethyl) furo [3,4-b] quinoxaline-1-one with o-phenylenediamine from non-purified CDs. On this basis, an AND-NAND logic pair was constructed and used as a ratiometric fluorescence sensor for highly sensitive detection of AA. The method has a wide linear range of 0.05-50 μM, and a detection limit of 34 nM. In addition, it was used to detect AA in fresh fruit. Potential applications include chemical computing, optoelectronic devices, biomedical science, and environmental monitoring. HIGHLIGHTS: 1. A ratiometric fluorescence sensor based on AND-NAND logic pair constructed by CDs and MoO₃ NSs was successfully fabricated. 2. The ratiometric fluorescence sensor exhibited satisfactory linear range, high sensitivity, and good selectivity for AA. 3. The ratiometric fluorescence method was able to detect AA in fresh fruit with good results comparable to official fluorescence methods.