Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Engineering of Curcumin-Based Lysosome-Targeting Photosensitizers for Boosting Tumor Photoimmunotherapy via Ferroptosis/Pyroptosis Activation and Autophagy Inhibition.

ACS nano·2026
Same author

Identification of α-tubulin alpha-1B chain as a target of asiatic acid using chemical proteomics in HepG2 hepatoma cells.

Organic & biomolecular chemistry·2024
Same author

The Development and Application of Tritium-Labeled Compounds in Biomedical Research.

Molecules (Basel, Switzerland)·2024
Same author

The Wdr5-H3K4me3 Epigenetic Axis Regulates Pancreatic Tumor Immunogenicity and Immune Suppression.

International journal of molecular sciences·2024
Same author

Bio-orthogonally activated tetraphenylene-tetrazine aggregation-induced emission fluorogenic probes.

Journal of materials chemistry. B·2022
Same author

Bioorthogonally applicable multicolor fluorogenic naphthalimide-tetrazine probes with aggregation-induced emission characters.

Chemical communications (Cambridge, England)·2021

Related Experiment Video

Updated: Oct 17, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.3K

A Dansyl Amide N-Oxide Fluorogenic Probe Based on a Bioorthogonal Decaging Reaction.

Hong Yang1, Yongcheng Wang1, Xiang Li1

  • 1Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, 100050, Beijing, China.

Chemistryopen
|October 12, 2021
PubMed
Summary

A novel "turn-on" fluorescence probe utilizing N-oxide chemistry was developed. This smart probe enables highly sensitive and selective bioimaging applications, including live-cell visualization.

Keywords:
N-oxidebioorthogonal decaging reactionscell imagingfluorogenic probesprotein labeling

More Related Videos

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

17.3K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.3K

Related Experiment Videos

Last Updated: Oct 17, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.3K
Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

17.3K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.3K

Area of Science:

  • Chemical Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Fluorescence probes are essential tools for biological research.
  • Developing probes with enhanced sensitivity and selectivity remains a key challenge.
  • Bioorthogonal reactions offer precise control for molecular labeling in complex biological systems.

Purpose of the Study:

  • To design and synthesize a smart fluorescence
  • turn-on" probe based on N-oxide chemistry.
  • To investigate the probe's reaction kinetics, fluorescence properties, and mechanism.
  • To evaluate the probe's performance in biological applications like protein labeling and live-cell imaging.

Main Methods:

  • Design and synthesis of a dansyl amide fluorophore-containing N-oxide probe.
  • Kinetic studies to determine reaction rates with boron reagents.
  • Time-dependent density functional theoretical (TD-DFT) calculations to elucidate the quenching mechanism.
  • Assessment of probe selectivity against amino acids and biocompatibility.
  • Application in HaloTag protein labeling and HepG2 live-cell imaging.

Main Results:

  • A rapid N-oxide decaging reaction with a boron reagent was achieved (k2 = 57.1±2.5 M−1 s−1).
  • The probe exhibited significant fluorescence enhancement (up to 72-fold) upon reaction.
  • Photoinduced electron transfer (PET) was identified as the fluorescence quenching mechanism.
  • High selectivity for nucleophilic amino acids and good biocompatibility were demonstrated.
  • Successful labeling of HaloTag proteins and imaging of HepG2 cells were accomplished.

Conclusions:

  • The developed smart fluorescence probe offers a sensitive and selective method for biomolecule visualization.
  • The probe's mechanism relies on bioorthogonal N-oxide decaging and PET.
  • This tool holds promise for advanced applications in chemical biology and live-cell imaging.