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C4 Pathway and CAM01:27

C4 Pathway and CAM

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Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
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What is Photosynthesis?00:39

What is Photosynthesis?

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Photosynthesis is a multipart, biochemical process that occurs in plants as well as in some bacteria. It captures carbon dioxide and solar energy to produce glucose. Glucose stores chemical energy in the form of carbohydrates. The overall biochemical formula of photosynthesis is 6 CO2 + 6 H2O + Light energy → C6H12O6 + 6 O2. Photosynthesis releases oxygen into the atmosphere and is largely responsible for maintaining the Earth’s atmospheric oxygen content.
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Photosystem I01:27

Photosystem I

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Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
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Photosystems01:32

Photosystems

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Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
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Photosystem II01:22

Photosystem II

75.1K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
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The Calvin Benson Cycle01:46

The Calvin Benson Cycle

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Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
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Testing the carbon starvation hypothesis using a late-successional neotropical tree species with green photosynthetic stems.

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High-temperature acclimation of photosystem II in land plants.

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Updating the occurrence of crassulacean acid metabolism (CAM) in the genus <i>Clusia</i> through carbon isotope analysis of species from Colombia.

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Related Experiment Video

Updated: Oct 17, 2025

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
10:46

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

Published on: December 9, 2022

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CAM photosynthesis: the acid test.

Klaus Winter1, J Andrew C Smith2

  • 1Smithsonian Tropical Research Institute, PO Box 0843-03092, Balboa, Ancón, Republic of Panama.

The New Phytologist
|October 12, 2021
PubMed
Summary

Nocturnal acidification, a key feature of crassulacean acid metabolism (CAM) photosynthesis, is not found in C3 plants. This suggests CAM evolved as a distinct innovation, not a simple enhancement of existing pathways.

Keywords:
C3 photosynthesisCAM photosynthesiscarboxylatecitratecrassulacean acid metabolismmalatemalic acidtitratable acidity

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Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
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Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
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Area of Science:

  • Plant Biology
  • Evolutionary Biology
  • Biochemistry

Background:

  • Crassulacean acid metabolism (CAM) photosynthesis enhances water-use efficiency.
  • Bioengineering CAM into C3 plants is of significant interest.
  • Understanding CAM evolution may reveal bioengineering targets.

Purpose of the Study:

  • Investigate the evolutionary origins of CAM photosynthesis.
  • Determine if nocturnal organic acid accumulation occurs in C3 plants.
  • Clarify the metabolic requirements for CAM evolution.

Main Methods:

  • Surveyed 40 families of vascular plants for nocturnal acidification.
  • Analyzed malate synthesis during light and dark periods.
  • Compared metabolic pathways of C3 and CAM plants.

Main Results:

  • Nocturnal acidification is exclusively observed in CAM species.
  • C3 plants can synthesize malate in the light but not at night.
  • CAM requires metabolic reprogramming for dark CO2 fixation.

Conclusions:

  • Nocturnal acidification is a unique CAM trait, not present in C3 plants.
  • CAM evolution involves fundamental metabolic reprogramming, not just flux enhancement.
  • CAM represents a discrete evolutionary innovation, not a continuum from C3.