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Sequestration of RBM10 in Nuclear Bodies: Targeting Sequences and Biological Significance
Ling-Yu Wang1,2, Sheng-Jun Xiao1,3, Hiroyuki Kunimoto1
1Department of Immunology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan.
International Journal of Molecular Sciences
|October 13, 2021
Summary
RNA-binding protein RBM10 localization to nuclear bodies (NBs) is regulated by its sequences and cellular transcription. RBM10
Area of Science:
- Molecular Biology
- Cell Biology
- RNA Biology
Background:
- RBM10 is an RNA-binding protein involved in alternative splicing (AS).
- RBM10 localizes to the nucleoplasm and S1-1 nuclear bodies (NBs).
Purpose of the Study:
- To investigate the biological significance of RBM10's localization to S1-1 NBs.
- To understand the relationship between RBM10's localization and its function in AS regulation.
Main Methods:
- Analysis of deletion mutants of RBM10.
- Investigation of RBM10 localization under varying cellular transcriptional activity.
Main Results:
- RBM10 possesses two synergistic NB-targeting sequences (NBTSs) in the KEKE motif and C2H2 Zn finger (ZnF).
- The C2H2 ZnF is crucial for both NB-targeting and AS regulation.
- RBM10 localization to S1-1 NBs increases as transcription declines, indicating it's a transient component sequestered when inactive.
Conclusions:
- RBM10 localization to S1-1 NBs is a dynamic process regulated by NBTSs and cellular transcription.
- The C2H2 ZnF plays a dual role in NB-targeting and AS regulation, potentially when RBM10 is not bound to pre-mRNAs.
- NB-localization serves as a mechanism to control RBM10's AS activity within the nucleus.
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