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Amyloid-β Processing in Aged S100B Transgenic Mice Is Sex Dependent
Krista Minéia Wartchow1,2, Leticia Rodrigues1,2, Izabela Swierzy2
1Department of Biochemistry, Federal University of Rio Grande do Sul, Porto Alegre 90035-003, Brazil.
Long-term elevated S100B protein levels in mice show sex-specific effects on amyloid-beta processing in the brain, suggesting a role in neurodegeneration.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- S100B protein's dual role in neuroregeneration and neurodegeneration is concentration/duration dependent.
- Investigated long-term S100B effects on amyloid-beta (Aβ) processing in transgenic mice.
Purpose of the Study:
- To examine the impact of sustained high S100B levels on Aβ processing.
- To consider sex and specific brain regions in this investigation.
Main Methods:
- Quantified S100B and Aβ42 in serum, CSF, adipose tissue, and brain regions using ELISA.
- Utilized Thioflavin T and Aβ immunostaining to visualize Aβ deposition.
Main Results:
- S100B levels were significantly elevated in serum, CSF, and brain of S100Btg mice (both sexes).
- Aβ42 increased in male hippocampus and female frontal cortex.
- Aβ deposition observed in various brain regions in S100Btg mice and female wild-type mice.
Conclusions:
- The study validates a model for S100B's role in neurodegeneration.
- Aβ processing is sex- and brain region-specific, warranting further research into signaling pathways and behavior.
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