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Updated: Oct 17, 2025

Pull-down of Calmodulin-binding Proteins
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Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase

Michał Marcinkowski1, Tomaš Pilžys1, Damian Garbicz1

  • 1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.

International Journal of Molecular Sciences
|October 13, 2021
PubMed

Insights

Fat mass and obesity-associated protein (FTO) interacts with calmodulin (CaM) in a calcium-dependent manner. This interaction influences CaM structure and suggests FTO

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Signaling

Background:

  • Fat mass and obesity-associated protein (FTO) is an N6-methyladenosine demethylase.
  • FTO is known to form complexes with other proteins, suggesting regulatory roles.
  • The specific regulatory proteins interacting with the FTO dioxygenase region are not fully characterized.

Purpose of the Study:

  • To identify regulatory proteins that interact with the FTO dioxygenase region.
  • To investigate the interaction between FTO and Calmodulin (CaM).
  • To elucidate the functional implications of the FTO-CaM interaction in calcium signaling.

Main Methods:

  • Bioinformatic analysis using the Calmodulin Target Database.
  • Experimental validation of FTO-CaM interaction.
  • Calcium-dependent binding assays.
  • Structural modeling of the FTO-CaM complex.

Main Results:

  • The FTO C-domain was identified as a potential CaM binding site.
  • Experimental evidence confirmed a calcium-dependent interaction between FTO and CaM.
  • The FTO-CaM interaction affects calcium-binding loops in CaM, indicating partial binding.
  • Structural modeling revealed a stable complex where Ca2+-saturated CaM interacts with the FTO C-domain.

Conclusions:

  • Calmodulin (CaM) is identified as a novel interactor of FTO.
  • The FTO-CaM interaction is calcium-dependent and influences CaM's structural dynamics.
  • These findings support the involvement of FTO in calcium signaling pathways.
Keywords:
FTOHDXMSTcalciumcalmodulin

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