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Osteosphere Model to Evaluate Cell-Surface Interactions of Implantable Biomaterials
Ana Carolina Batista Brochado1,2, Victor Hugo de Souza1,3, Joice Correa2
1Post-Graduation Program in Science & Biotechnology, Institute of Biology, Fluminense Federal University, Niteroi 24210-201, Brazil.
Materials (Basel, Switzerland)
|October 13, 2021
Summary
A novel 3D osteosphere model effectively evaluates bone implant surfaces. Sandblasted and acid-etched titanium surfaces show superior osteoconductive properties compared to hydroxyapatite-coated surfaces.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Successful bone tissue therapy requires biomaterials that promote osteogenic cell migration, proliferation, and attachment.
- Implant stability is crucial to avoid post-surgical complications.
Purpose of the Study:
- To evaluate the osteoconductive properties of different titanium implant surfaces using a novel three-dimensional (3D) osteosphere model.
- To assess cell-surface interactions, including proliferation, migration, and spreading.
Main Methods:
- Three titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA).
- Surface characterization using Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM).
- Seeding osteospheres onto surfaces and analyzing cell-surface interactions.
Main Results:
- BE surfaces exhibited higher cell densities and greater evidence of cell migration compared to MA surfaces.
- PSHA surfaces demonstrated the lowest performance across all analyzed parameters.
- The 3D osteosphere model facilitated focal analysis of in vitro cell/surface interactions.
Conclusions:
- The 3D osteosphere model is a valuable tool for evaluating the osteoconductive properties of biomaterials.
- BE surfaces show promising potential for bone tissue engineering applications.
- This model may serve as a predictive preclinical tool for novel biomaterial development.
