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Updated: Oct 17, 2025

Electrochemiluminescence Assays for Human Islet Autoantibodies

Electrochemiluminescence Assays for Human Islet Autoantibodies

Published on: March 23, 2018

Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter.

Hyogu Han^1,2, Junhyun Park^1,3, Jun Ki Ahn¹

¹Material & Component Convergence R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, Korea.

Sensors (Basel, Switzerland)

|October 13, 2021

Summary

A novel cascade enzymatic reaction (CER) method uses a personal glucose meter (PGM) for sensitive IgE detection. This approach offers a portable, cost-effective alternative to ELISA for allergy diagnostics.

Keywords:

alkaline phosphatase biosensor cascade enzymatic reaction immunoglobulin E personal glucose meter

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Last Updated: Oct 17, 2025

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Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Published on: May 10, 2018

Area of Science:

Biochemistry and Analytical Chemistry
Immunodiagnostics
Point-of-Care Testing

Background:

Accurate and accessible detection of Immunoglobulin E (IgE) is crucial for diagnosing allergic diseases.
Current methods like ELISA are effective but require specialized equipment and trained personnel, limiting point-of-care applications.
There is a need for simplified, portable, and cost-effective IgE detection systems.

Purpose of the Study:

To develop a novel cascade enzymatic reaction (CER)-based assay for IgE detection.
To utilize a personal glucose meter (PGM) for quantifying IgE levels, enabling point-of-care use.
To evaluate the sensitivity, specificity, and diagnostic capability of the developed method.

Main Methods:

A sandwich assay format was employed to capture target IgE, incorporating alkaline phosphatase (ALP) activity.
ALP activity regulated adenosine triphosphate (ATP) levels, which served as a substrate for the CER.
Changes in glucose levels, catalyzed by CER, were measured using a standard personal glucose meter (PGM).

Main Results:

The method achieved a limit of detection (LOD) as low as approximately 29.6 ng/mL for IgE.
High specificity was demonstrated against various proteins, confirming assay accuracy.
The PGM-based approach showed comparable LOD to commercial ELISA kits but without complex equipment or washing steps.

Conclusions:

The developed CER-based IgE detection method using a PGM is sensitive, specific, and portable.
This assay offers a cost-effective and simplified alternative to traditional ELISA for IgE quantification.
The method's diagnostic capability was validated in real human serum samples with excellent recovery (100 ± 6%).