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Development of Gelatin-Coated Microspheres for Novel Bioink Design
Muskan Kanungo1, Yale Wang2, Noah Hutchinson3
1Biomolecular Engineering Program, Physics and Chemistry Department, Milwaukee School of Engineering, Milwaukee, WI 53202, USA.
Polymers
|October 13, 2021
Summary
Positively charged gelatin-coated pectin microspheres were developed for tissue engineering bioinks. These microspheres promote vascularization and controlled release, showing promise for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Vascularization is a critical challenge in tissue engineering.
- Positively charged microspheres can enhance vascularization and controlled release of bioactive molecules.
Purpose of the Study:
- To develop gelatin-coated pectin microspheres for bioink applications.
- To optimize microsphere production and characterize their properties for tissue engineering.
Main Methods:
- Electrospray technique was employed for microsphere fabrication.
- Design-Expert software was used for process optimization.
- Microspheres were modified with gelatin coating and EDC catalysis.
Main Results:
- Pectin concentration influenced microsphere roundness and uniformity.
- Flow rate was the primary factor affecting microsphere size.
- Optimal gelatin coating (0.75%) resulted in a positively charged surface.
- Incorporated microspheres did not alter bioink viscosity and distributed evenly.
Conclusions:
- Gelatin-coated pectin microspheres are suitable for bioink formulation.
- These microspheres hold potential for enhancing vascularization in engineered tissues.
- The developed microspheres offer a promising platform for regenerative medicine strategies.
