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A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone
Sutasinee Apichai1,2,3, Kajorngai Thajee3, Thanawat Pattananandecha1,2,3
1Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Molecules (Basel, Switzerland)
|October 13, 2021
Summary
A novel antioxidant analysis uses a simple device and smartphone to measure DPPH radical scavenging activity. This method accurately quanties antioxidant capacity, offering a cost-effective alternative to traditional spectrophotometry.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Antioxidant analysis is crucial for evaluating bioactive compounds.
- Traditional methods like spectrophotometry can be expensive and require specialized equipment.
- A need exists for simple, portable, and cost-effective antioxidant detection systems.
Purpose of the Study:
- To propose a novel antioxidant analysis method using a minimized device and smartphone.
- To determine the scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•).
- To evaluate antioxidant capacity using metrics like IC50, VCEAC, and TEAC.
Main Methods:
- Utilized a simple device with moving drops for sample handling.
- Employed a smartphone camera as a detector for colorimetric analysis.
- Quantified DPPH• inhibition using the magenta-to-yellow color ratio processed via Adobe Photoshop.
- Measured total phenolic content (TPC) using the Folin-Ciocalteu assay.
Main Results:
- The smartphone-based method showed no significant difference compared to the reference spectrophotometric method (95% confidence level).
- The magenta-to-yellow ratio effectively evaluated DPPH• inhibition percentage.
- Demonstrated a consonant relationship between DPPH• scavenging activity and TPC in Miang (fermented tea leaf extract).
Conclusions:
- The proposed method offers a simple, cost-effective, and accurate approach for antioxidant analysis.
- Smartphone-based colorimetric detection is a viable alternative to conventional spectrophotometry.
- The method is applicable for analyzing antioxidant properties in real-world samples like fermented tea extracts.
