Vaccinia virus DNA sequences in the nucleus of persistently infected Friend erythroleukemia cells

FL vac cell lines are Friend erythroleukemia cells persistently infected with vaccinia virus. These cells produce attenuated leukemia virus, virulent poxvirus, resist superinfection with vaccinia, and show high levels of spontaneous erythrodifferentiation and decreased tumorigenicity in syngeneic hosts (Pogo, G.T. and Friend, C. (1982) Proc. Natl. Acad. Sci. USA 79, 4805-4809). To determine whether resistance to superinfection was associated with the presence of vaccinia DNA in the nucleus, DNA from cells at different passage levels was hybridized to a vaccinia DNA probe. Vaccinia DNA sequences were detected in the nucleus of cells of lines that were productively infected with vaccinia. No such sequences were detected in productively infected with vaccinia. No such sequences were detected in productively infected L cells nor in persistently infected cell lines that no longer produced infectious particles but were resistant to superinfection. Although no evidence of integration of vaccinia DNA was observed, differences in the restriction patterns were detected at some passage levels. The presence of vaccinia virus DNA sequences in the nucleus apparently did not affect the size of the provirus, the integration pattern or the expression of the leukemia virus.