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Updated: Oct 17, 2025
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
A Simple and Sensitive Nanogold RRS/Abs Dimode Sensor for Trace As3+ Based on Aptamer Controlled Nitrogen Doped
Hongyan Bai1,2, Haolin Wang1,2, Fuzhang Bai1,2
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China.
Abstract:
Using citric acid (CA) and ethylenediamine (EDA) as precursors, stable nitrogen-doped carbon dots (CD) nanosols were prepared by microwave procedure and characterized in detail. It was found that CDNs catalyze ethanol (Et)-HAuCl4 to generate gold nanoparticles (AuNPs), which have strong surface plasmon resonance, Rayleigh scattering, (RRS) and a surface plasmon resonance (SPR) absorption (Abs) effect at 370 nm and 575 nm, respectively. Compled the new catalytic amplification indicator reaction with the specific As3+ aptamer reaction, a new RRS/Abs dual-mode aptamer sensor for the assay of trace As3+ was developed, based on the RRS/Abs signals increasing linearly with As3+ increasing in the ranges of 5-250 nmol/L and 50-250 nmol/L, whose detection limits were 0.8 nmol/L and 3.4 nmol/L As3+, respectively. This analytical method has the advantages of high selectivity, simplicity, and rapidity, and it has been successfully applied to the detection of practical samples.
