HBFP: a new repository for human body fluid proteome

Area of Science:

• Biochemistry
• Proteomics
• Bioinformatics

Background:

• Body fluid proteome analysis is crucial for disease biomarker discovery.
• Existing resources for body fluid proteins are fragmented and lack systematic tools.
• A centralized, sustainable repository is needed for comprehensive analysis.

Purpose of the Study:

• To develop a new database of human body fluid proteome (HBFP).
• To provide a centralized resource for experimentally validated proteins from 17 human body fluids.
• To offer systematic analytic tools for querying, analyzing, and downloading protein data.

Main Methods:

• Compiled data from 164 scientific publications since 2001.
• Included proteins with a maximal false discovery rate of 0.01.
• Developed annotation pages with abundance information and a confidence score.
• Cataloged proteins based on Human Proteome Project Data Interpretation Guidelines.

Main Results:

• The HBFP database archives 11,827 unique proteins.
• Proteins are categorized by 17 body fluid types.
• Detailed annotation includes peptide abundance and a novel confidence score.
• 7354 proteins meet Human Proteome Project criteria for unique mapping peptides.

Conclusions:

• The HBFP database serves as a vital resource for the human protein secretome.
• It facilitates research in clinical proteomics and accelerates biomarker discovery.
• The database provides a powerful tool for systematic analysis of body fluid proteomic data.