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Updated: Oct 17, 2025

A Standardized Protocol for Preference Testing to Assess Fish Welfare
Published on: February 22, 2020
A Psychological Aversive Condition Does Not Change Individual Zebrafish Preference For Background Color Or Artificial
Caroline Marques Maia1, Gilson Luiz Volpato1, Victoria Anne Braithwaite2
1Laboratory of Animal Physiology and Behavior, Institute of Biosciences (Ib), Unesp, Botucatu, SP, Brazil.
Abstract:
Information on fish preference for environmental conditions can be a useful tool to offer them what they want, an important requirement for welfare purposes. Giving that such an approach deals with psychological states of the fish, we investigated whether psychological stress affects fish preference. In this study, we found that the aversive condition of crossing a white open field did not change individual zebrafish's preference for color background or for plant enrichments, despite a great individual variability of preference response. We conclude that individual preferences for environmental conditions are maintained even following a psychological stressor, and highlight that variability in preference options among individuals raises questions about how to best improve welfare of grouped zebrafish.
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