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Procoagulant activity of activated monocytes
Abstract:
Endotoxin-stimulated monocytes develop a very high tissue-factor-like activity as compared to an equivalent number of monocytes in a preparation of monocyte-enriched mononuclear blood cells obtained by Ficoll-Urovision density gradient centrifugation which is not exposed to endotoxin. In monocytes which were obtained by plastic adherence from the monocyte-enriched mononuclear blood cells, the tissue factor activity was as high as in the endotoxin-stimulated monocytes.
Insights
Endotoxin exposure significantly increases tissue factor activity in monocytes. Monocytes isolated by plastic adherence exhibit high tissue factor activity, similar to endotoxin-stimulated cells.
Area of Science:
- Immunology
- Hematology
Background:
- Monocytes play a crucial role in hemostasis and thrombosis.
- Tissue factor is a key initiator of the coagulation cascade.
Purpose of the Study:
- To investigate the effect of endotoxin stimulation on monocyte tissue factor activity.
- To compare tissue factor activity in monocytes isolated by different methods.
Main Methods:
- Monocyte-enriched mononuclear blood cells were isolated using Ficoll-Urovision density gradient centrifugation.
- Tissue factor-like activity was measured in monocytes with and without endotoxin stimulation.
- Monocytes were also isolated by plastic adherence for activity assessment.
Main Results:
- Endotoxin-stimulated monocytes exhibited significantly higher tissue factor activity compared to unstimulated monocytes.
- Monocytes isolated by plastic adherence showed tissue factor activity comparable to endotoxin-stimulated monocytes.
- This suggests that plastic adherence may induce or reveal high tissue factor activity.
Conclusions:
- Monocyte tissue factor activity is markedly enhanced by endotoxin stimulation.
- Plastic adherence is an effective method for isolating monocytes with high intrinsic tissue factor activity.
- These findings have implications for understanding monocyte procoagulant potential in various physiological and pathological conditions.