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circAMOTL1L Suppresses Renal Cell Carcinoma Growth by Modulating the miR-92a-2-5p/KLLN Pathway

Ling Gao1, Xian Shao2, Qingqing Yue3

  • 1Department of Oncology, Kaifeng Central Hospital, Kaifeng, Henan, China.

Oxidative Medicine and Cellular Longevity
|October 14, 2021
PubMed

Insights

Circular RNAs (circRNAs) like circAMOTL1L are downregulated in renal cell carcinoma (RCC). circAMOTL1L inhibits RCC progression by regulating the miR-92a-2-5p-KLLN pathway, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly implicated in cancer progression.
  • The specific roles of circRNAs in renal cell carcinoma (RCC) are not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of circAMOTL1L in RCC.
  • To determine if circAMOTL1L has a role in tumor suppression in RCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess circAMOTL1L expression.
  • In vitro cell proliferation and apoptosis assays.
  • In vivo xenograft tumor growth studies in nude mice.
  • Bioinformatics analysis, oligo pull-down, and luciferase assays to elucidate molecular mechanisms.

Main Results:

  • circAMOTL1L was significantly downregulated in RCC tissues and cell lines, correlating with advanced tumor stage, metastasis, and poor prognosis.
  • Overexpression of circAMOTL1L suppressed RCC cell proliferation and induced apoptosis.
  • circAMOTL1L acted as a molecular sponge for miR-92a-2-5p, leading to the upregulation of KLLN (killin) expression.
  • Restoration of the circAMOTL1L-miR-92a-2-5p-KLLN axis inhibited tumor growth in vivo.

Conclusions:

  • circAMOTL1L exhibits antioncogenic properties in RCC.
  • The circAMOTL1L-miR-92a-2-5p-KLLN pathway is a key regulatory axis in RCC progression.
  • This pathway represents a potential therapeutic target for RCC treatment.

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