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Updated: Oct 17, 2025
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
An optimized ATAC-seq protocol for genome-wide mapping of active regulatory elements in primary mouse cortical
Maya Maor-Nof1, Zohar Shipony1, Georgi K Marinov1
1Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Abstract:
ATAC-seq is a versatile, adaptable, and widely adopted technique for mapping open chromatin regions. However, some biological systems, such as primary neurons, present unique challenges to its application. Conventional ATAC-seq would require the dissociation of the primary neurons after plating but dissociating them leads to rapid cell death and major changes in cell state, affecting ATAC-seq results. We have developed this modified ATAC-seq protocol to address this challenge for primary neurons, providing a high-quality and high-resolution accessible chromatin profile. For complete details on the use and execution of this protocol, please refer to Maor-Nof et al. (2021).
