Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Retraction notice to "Dual Z-scheme core-shell PANI-CeO<sub>2</sub>-Fe<sub>2</sub>O<sub>3</sub>-NiO heterostructured nanocomposite for dyes remediation under sunlight and bacterial disinfection" [Environ. Res. 215 (2022) 114140].

Environmental research·2026

Same author

A low-cost color sensor device for rapid detection of high-grade serous ovarian carcinoma (HGSOC).

Oncoscience·2026

Same author

Retraction notice to "Boosted charge separation via Ce<sub>2</sub>S<sub>3</sub> over dual Z-scheme ZnO-Ce<sub>2</sub>S<sub>3</sub>-MnO<sub>2</sub> core double-shell nanocomposite for the degradation of diverse dye pollutants" [Environ. Res. 251 (2024) 118675].

Environmental research·2026

Same author

A Comparative Analysis of Clinical Features of Type 2 Diabetes Mellitus With Respect to Dyslipidemia: A Cross-Sectional Study.

Cureus·2026

Same author

Corrigendum to "Investigation of synergistically developed p-Co<sub>3</sub>O<sub>4</sub>/n-ZnHoCuO based junction for enhanced photocatalytic, electrocatalytic, and energy storage applications" [J. Colloid Interface Sci. 699(Part 1) (2025) 138045].

Journal of colloid and interface science·2025

Same author

Bridging clinical insight and laboratory model in high-grade serous ovarian carcinoma (HGSOC) using DNA sequencing-based profiling of TP53.

Oncoscience·2025

Search research articles

Related Experiment Video

Updated: Oct 17, 2025

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Genetic analysis with pyrosequencing using loop pipetting and a light dependent resistor.

Faisal Iqbal¹, Muhammad Imran Shabbir¹

¹Department of Biological Sciences, Faculty of Basic & Applied Sciences, International Islamic University, Sector H-10, Islamabad, Pakistan. imran.shabbir@iiu.edu.pk.

Analytical Methods : Advancing Methods and Applications

|October 14, 2021

Summary

A new, low-cost DNA sequencing device, LoopSeeq, offers accurate pyrosequencing for genetic analysis. This portable technology makes DNA sequencing accessible for remote diagnostics and routine labs, overcoming the limitations of expensive, bulky equipment.

More Related Videos

Pyrosequencing for Microbial Identification and Characterization

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

Related Experiment Videos

Last Updated: Oct 17, 2025

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Pyrosequencing for Microbial Identification and Characterization

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Conventional DNA sequencing instruments are expensive, bulky, and require trained personnel, limiting their accessibility in many laboratories and remote settings.
There is a need for cost-effective, portable, and user-friendly DNA sequencing solutions for broader application in health, disease, and diagnostics.

Purpose of the Study:

To develop and demonstrate a proof-of-concept for a low-cost, portable DNA sequencing device named LoopSeeq.
To address the accessibility issues associated with current DNA sequencing technologies.

Main Methods:

LoopSeeq utilizes pyrosequencing with iterative deoxynucleotide triphosphate (dNTP) addition via a loop pipette and chemiluminescence detection using a light-dependent resistor (LDR).
The device is controlled by an Arduino Nano microcontroller, geared motors, and transfers real-time data to a laptop for analysis.
Pyrosequencing was optimized using a 55 nt self-primed oligo, and its performance was validated by analyzing a novel mutation in the OCA2 gene.

Main Results:

LoopSeeq successfully performed pyrosequencing and accurately identified homozygous normal, homozygous mutant, and heterozygous alleles for a specific OCA2 gene mutation in a family with oculocutaneous albinism.
The device demonstrated the potential for molecular genetic analysis in real-world samples, including pathogen detection and genotyping.

Conclusions:

The LoopSeeq device presents a viable, low-cost alternative for DNA sequencing, suitable for routine laboratories and challenging real-world scenarios like point-of-care and remote diagnostics.
Future designs should address limitations such as reagent evaporation and loopette contamination for extended use.