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Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies
Alexander Meyers1,2, Nathan Scinto-Madonich3, Sarah E Wyatt1,2
1Department of Environmental & Plant Biology, Ohio University, Athens, OH, USA.
Abstract:
Polyethersulfone (PES) membranes provide a versatile tool for gravity-related plant studies. Benefits of this system include straightforward setup, no need for specialized equipment, long-term seed viability between plating and hydration/growth, and adaptability to diverse protocols and downstream analyses. Methods outlined here include seed sterilization, planting, growth, and dissection that will transition directly into any RNA extraction protocol.
