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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
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m6A regulator-mediated RNA methylation modification patterns and immune microenvironment infiltration
Deyang Sun1, Huan Yang1, Liming Fan1
1The First Clinical College, Zhejiang Chinese Medical University, Hangzhou, China.
Journal of Cellular and Molecular Medicine
|October 14, 2021
Summary
N6-methyladenosine (m6A) RNA modification patterns are altered in severe asthma, impacting immune cell infiltration. Key regulators like YTHDF3 and YTHDC1 offer potential targets for future immunotherapy strategies.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Background:
- N6-methyladenosine (m6A) is a prevalent mRNA modification regulating gene expression.
- The role of m6A modification in severe asthma pathogenesis remains unexplored.
Purpose of the Study:
- To investigate m6A regulator-mediated RNA methylation patterns in severe asthma.
- To characterize immune microenvironment infiltration in relation to m6A patterns.
Main Methods:
- Analysis of 27 m6A regulators in 87 healthy controls and 344 severe asthma cases (U-BIOPRED cohort).
- Systematic evaluation of m6A patterns and immune cell infiltration.
- Validation of key regulators using merip-seq.
Main Results:
- 16 m6A regulators showed abnormal expression in severe asthma.
- Two key regulators (YTHDF3, YTHDC1) and three distinct m6A patterns were identified.
- Pattern 2 was associated with increased immune cell infiltration and activity, and eosinophil levels were linked to YTHDF3 and EIF3B.
Conclusions:
- m6A modification plays a significant role in severe asthma.
- Identified m6A regulators and patterns may guide future immunotherapy strategies for severe asthma.
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