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Updated: Oct 17, 2025

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation
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A tripartite carbohydrate-binding module to functionalize cellulose nanocrystals.

Angeline Pelus1, Gaëlle Bordes2, Sophie Barbe1

  • 1TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France. cedric.montanier@insa-toulouse.fr.

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Researchers engineered a versatile protein to add diverse functions to cellulose, a renewable biomaterial. This strategy enhances cellulose

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Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Biotechnology

Background:

  • Cellulose, a renewable polysaccharide, has unique properties for industrial applications.
  • Current cellulose functionalization methods are limited in scope and diversity.
  • Biotechnology offers potential for developing novel biomaterials from renewable resources.

Purpose of the Study:

  • To develop a versatile strategy for multi-functionalizing cellulose surfaces.
  • To engineer a tripartite chimeric protein using a Carbohydrate-Binding Module (CBM) as a core.
  • To demonstrate the potential of this engineered protein for diverse applications.

Main Methods:

  • Utilized CBM3a from *Clostridium thermocellum* for high affinity to crystalline cellulose.
  • Engineered a tripartite chimeric protein with streptavidin and azide functionalities.
  • Employed molecular modeling for structural analysis.
  • Experimentally validated multi-functionalization of cellulose surfaces.

Main Results:

  • Successfully produced and functionalized the tripartite chimeric protein.
  • Demonstrated the protein's ability to bind to cellulose surfaces.
  • Confirmed multi-functionalization potential through experimental validation.
  • Showcased proof-of-concept using fluorescently labeled protein on magnetic beads.

Conclusions:

  • The engineered tripartite chimeric protein provides a versatile platform for cellulose multi-functionalization.
  • This approach significantly expands the functional capabilities of cellulose-based biomaterials.
  • The strategy holds promise for advancing sustainable biomaterial development and applications.