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Updated: Oct 16, 2025

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
Published on: January 26, 2024
Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
Masahiko Takemura1, Yi-Si Lu1, Eriko Nakato1
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis USA.
Abstract:
Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in Drosophila. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the upd1 gene. Anti-HA antibody staining confirms that the distribution of the epitope-tagged Upd1::HA in various tissues is consistent with upd1 expression patterns revealed by previous studies. This transgenic fly strain will be useful in studying the expression, localization, and association partners of Upd1, and thus will contribute to understanding how activation of the JAK/STAT pathway is regulated.
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