Related Experiment Video
Updated: Oct 16, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Variety-specific transcript accumulation during reproductive stage in drought-stressed rice
Pratibha Gour1, Shivani Kansal1, Priyanka Agarwal1
1Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
Rice varieties exhibit distinct drought responses. Drought-tolerant Nagina 22 showed more differentially expressed genes (DEGs) than sensitive IR64, revealing key genes and pathways for drought tolerance.
Area of Science:
- Plant Biology
- Genomics
- Stress Physiology
Background:
- Natural variation in stress tolerance mechanisms between species is significant.
- Understanding these differences in rice can improve crop resilience.
Purpose of the Study:
- To comparatively analyze gene expression in drought-tolerant (Nagina 22) and drought-sensitive (IR64) rice varieties under field drought.
- To identify key genes and pathways involved in differential drought tolerance.
Main Methods:
- Comparative transcriptome analysis of flag leaf, panicles, and roots at the heading stage.
- Co-expression network analysis to identify hub genes.
- Annotation and pathway analysis of differentially expressed genes (DEGs).
Main Results:
- Nagina 22 exhibited nearly twice the number of DEGs compared to IR64 under drought.
- DEGs were enriched in drought-related quantitative trait loci (QTLs) and associated with traits like leaf rolling and sugar content.
- 1366 variety-specific DEGs (VS-DEGs), including 21 hub genes, showed opposite regulation between the varieties.
- VS-DEGs participate in diverse biological pathways and exhibit complex gene interactions.
Conclusions:
- Significant differences in gene expression patterns underlie the distinct drought tolerance of rice varieties.
- Promoter sequence variations may contribute to the unique expression profiles of VS-DEGs.
- This study identifies potential targets for breeding more drought-resilient rice cultivars.
More Related Videos
15:30A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions
Published on: August 5, 2020
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
Related Concept Videos
Responses to Drought and Flooding
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Adaptations that Reduce Water Loss
Gene Regulation During Sporulation