PCR-based next-generation West Nile virus sequencing protocols
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Updated: Oct 16, 2025
Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
Styliani Pappa1, Serafeim C Chaintoutis2, Chrysostomos I Dovas2
1National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
Abstract:
The epidemiology of West Nile virus (WNV) is unpredictable and changing. Availability of whole genome sequences enables the detailed molecular epidemiology studies and the evaluation and design of diagnostic tools. In the present study we provide two PCR-based protocols which can be applied directly on biological samples from hosts infected by WNV strains belonging to lineage 1 or lineage 2. It was shown that the protocols worked successfully even on samples with relatively low viral load.
