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PCR-based next-generation West Nile virus sequencing protocols.

Styliani Pappa1, Serafeim C Chaintoutis2, Chrysostomos I Dovas2

  • 1National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.

Molecular and Cellular Probes
|October 15, 2021
PubMed
Summary
This summary is machine-generated.

West Nile virus (WNV) molecular epidemiology is evolving. New PCR protocols accurately detect WNV lineages 1 and 2 directly from host samples, even with low viral loads.

Keywords:
Next generation sequencingWest nile virusWhole genome

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Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Background:

  • West Nile virus (WNV) epidemiology is dynamic and unpredictable.
  • Whole genome sequencing aids in detailed molecular epidemiology and diagnostic tool development.
  • Understanding WNV strain diversity is crucial for effective surveillance and control.

Purpose of the Study:

  • To develop and validate novel PCR-based protocols for WNV detection.
  • To enable direct application of protocols on biological samples from infected hosts.
  • To target WNV strains belonging to both lineage 1 and lineage 2.

Main Methods:

  • Development of two distinct PCR-based protocols.
  • Direct application of protocols on biological samples (e.g., blood, tissue).
  • Testing protocol efficacy on samples infected with WNV lineage 1 and lineage 2 strains.

Main Results:

  • Successful implementation of two PCR protocols for WNV detection.
  • Protocols demonstrated effectiveness on samples with varying viral loads, including low viral concentrations.
  • Validated detection of both WNV lineage 1 and lineage 2.

Conclusions:

  • The developed PCR protocols offer a reliable method for WNV molecular epidemiology.
  • These tools facilitate direct detection from host samples, simplifying diagnostics.
  • The protocols are valuable for surveillance and research of WNV, particularly for diverse lineages.