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Direct measurement of nitric oxide (NO) production rates from enzymes using ozone-based gas-phase chemiluminescence

Courtney E Sparacino-Watkins1, Jack R Lancaster2

  • 1Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261, USA.

Nitric Oxide : Biology and Chemistry
|October 15, 2021
PubMed

Keywords:
ChemiluminescenceKineticsNitric oxideNitric oxide analyzerNitrite

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Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...