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Recent technological developments for native mass spectrometry.

Ian K Webb1

  • 1Department of Chemistry and Chemical Biology, Purdue School of Science, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, United States of America; Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, United States of America.

Biochimica Et Biophysica Acta. Proteins and Proteomics
|October 15, 2021
PubMed
Summary
This summary is machine-generated.

Native mass spectrometry (MS) analyzes proteins in their native structures. Advances in gas-phase instrumentation enable sensitive, selective analysis for structural biology and proteomics.

Keywords:
Ion chemistryIon mobilityNative mass spectrometryTandem mass spectrometry

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Area of Science:

  • Structural biology
  • Biochemistry
  • Analytical chemistry

Background:

  • Native mass spectrometry (MS) analyzes proteins and protein complexes in solution, preserving native structures.
  • Proteins maintain native folds in the gas phase under MS experimental conditions.
  • Mass spectrometry offers speed, sensitivity, and selectivity for biological analyses.

Purpose of the Study:

  • To review recent advances in gas-phase instrumentation for native mass spectrometry.
  • To highlight applications of these advancements in structural proteomics.

Main Methods:

  • Analysis of proteins and protein complexes using native mass spectrometry.
  • Development of specialized instrumentation for gas-phase native-like ion analysis.
  • Exploitation of MS speed, sensitivity, and selectivity.

Main Results:

  • Strong evidence supports the retention of native protein folds in the gas phase.
  • Instrumentation has been developed to utilize gas-phase native-like protein ions.
  • These advancements address emerging problems in structural biology.

Conclusions:

  • Gas-phase native mass spectrometry is a rapidly expanding area.
  • Recent instrumentation advances enhance the application of MS in structural proteomics.
  • This technique provides powerful tools for understanding protein structure and function.