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Updated: Oct 16, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
Published on: April 13, 2022
Protein dynamics implications of the low- and high-temperature denaturation of myoglobin
Ramzi R Khuri1, Trung V Phan2, Robert H Austin2
1Department of Natural Sciences, Baruch College, City University of New York, New York, New York 10010, USA.
Abstract:
We reinvestigate a simple model used in the literature concerning the thermodynamic analysis of protein cold denaturation. We derive an exact thermodynamic expression for cold denaturation and give a better approximation than exists in the literature for predicting cold denaturation temperatures in the two-state model. We discuss the "dark-side" implications of this work for previous temperature-dependent protein dynamics experiments and discuss microfluidic experimental technologies, which could explore the thermal stability range of proteins below the bulk freezing point of water.
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