Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11...

Area of Science:

• Reproductive Biology
• Molecular Biology
• Cell Biology

Background:

• Recurrent pregnancy loss (RPL) is linked to impaired trophoblastic cell function.
• Circular RNAs (circRNAs) are implicated in various diseases, but their role in RPL is unclear.
• Circular RNA forkhead box P1 (circFOXP1) is a potential regulator of cellular processes.

Purpose of the Study:

• To investigate the role of circFOXP1 in trophoblastic cells and its involvement in recurrent pregnancy loss.
• To elucidate the molecular mechanism of circFOXP1 in regulating trophoblastic cell function.
• To identify potential biomarkers for RPL.

Main Methods:

• Gene and protein expression analysis (RT-qPCR, Western blotting).
• Assessment of trophoblastic cell viability, apoptosis, invasion, and migration (CCK-8, flow cytometry, wound healing, Transwell assays).
• Bioinformatics prediction, luciferase reporter assays, and RNA immunoprecipitation to confirm molecular interactions.

Main Results:

• circFOXP1 was found to regulate trophoblastic cell function via the miR-143-3p/S100A11 axis.
• miR-143-3p upregulation in RPL induced apoptosis and suppressed proliferation and epithelial-to-mesenchymal transition (EMT).
• circFOXP1 competitively bound to miR-143-3p, thereby regulating S100A11 expression and trophoblastic cell functions.

Conclusions:

• circFOXP1 enhances trophoblastic cell function by modulating the miR-143-3p/S100A11 pathway.
• This circFOXP1/miR-143-3p/S100A11 axis represents a novel therapeutic and diagnostic target for recurrent pregnancy loss.
• The study provides new insights into the molecular mechanisms underlying RPL.