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Updated: Oct 16, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

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LncRNA FGF14-AS2 represses growth of prostate carcinoma cells via modulating miR-96-5p/AJAP1 axis

Rubing Li1, Yingcong Chen2, Jingwei Wu2

  • 1Department of Urology, Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang, China.

Journal of Clinical Laboratory Analysis
|October 16, 2021
PubMed
Abstract

Insights

Long non-coding RNA FGF14-AS2 is downregulated in prostate cancer. It inhibits cancer progression by regulating miR-96-5p and AJAP1, impacting cell proliferation and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Prostate carcinoma is a significant health concern with complex progression mechanisms.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of lncRNA FGF14 antisense RNA 2 (FGF14-AS2) in prostate cancer remains underexplored.

Purpose of the Study:

  • To investigate the role of lncRNA FGF14-AS2 in prostate carcinoma progression.
  • To elucidate the molecular mechanism involving FGF14-AS2, miR-96-5p, and Adherens junction-associated protein-1 (AJAP1).

Main Methods:

  • Quantitative real-time PCR and Western blot were used to assess expression levels of FGF14-AS2, miR-96-5p, and AJAP1.
  • RNA immunoprecipitation and dual-luciferase assays confirmed interactions within the FGF14-AS2/miR-96-5p/AJAP1 axis.
  • In vitro (MTT, Transwell) and in vivo (nude mice tumor model) experiments evaluated the functional impact of this axis on prostate cancer progression.

Main Results:

  • FGF14-AS2 was found to be downregulated in prostate carcinoma tissues and cells.
  • FGF14-AS2 suppressed miR-96-5p expression, which in turn inhibited AJAP1.
  • Overexpression of FGF14-AS2 reduced prostate cancer cell proliferation and metastasis, while its knockdown had opposite effects. miR-96-5p mimic promoted cancer progression.
  • AJAP1 levels influenced proteins associated with epithelial-mesenchymal transition. In vivo studies confirmed FGF14-AS2's ability to inhibit metastasis and tumor growth by modulating AJAP1.

Conclusions:

  • lncRNA FGF14-AS2 is downregulated in prostate cancer and acts as a tumor suppressor.
  • FGF14-AS2 influences prostate cancer cell proliferation and metastasis through the miR-96-5p/AJAP1 pathway.
  • Targeting the FGF14-AS2/miR-96-5p/AJAP1 axis may offer therapeutic strategies for prostate carcinoma.
Keywords:
AJAP1FGF14-AS2miR-96-5pprogressionprostate carcinoma

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