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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
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Microbial community alteration in tongue squamous cell carcinoma
Peng Ye1, Ye Liu2,3, Ye-Jun Cai1
1Department of Stomatology, Beijing Hospital, National Center of Gerontology, National Health Commission, Institute of Geriatric Medicine, Chinese Academy of Medical Science, Beijing, 100730, China.
Applied Microbiology and Biotechnology
|October 16, 2021
Summary
The oral microbiome in tongue squamous cell carcinoma (TSCC) shows increased diversity and altered bacterial composition compared to normal tissues. These findings highlight the microbiome's role in TSCC development and progression.
Area of Science:
- Microbiology
- Oncology
- Oral Health
Background:
- Tongue squamous cell carcinoma (TSCC) is a prevalent oral malignancy.
- The specific role of the oral microbial community in TSCC pathogenesis remains largely undefined.
Purpose of the Study:
- To investigate alterations in the oral microbial community associated with TSCC.
- To explore the potential link between microbial dysbiosis and TSCC development.
Main Methods:
- Recruited 23 patients with TSCC, collecting tissue DNA from cancerous and adjacent normal tissues.
- Utilized next-generation 16S rDNA amplicon sequencing for taxonomic and functional analysis.
- Applied diversity indexes (Shannon, Simpson, OTUs, Chao1) and community structure analyses (Adonis, ANOSIM).
Main Results:
- Cancerous tissues exhibited significantly higher microbial diversity (Shannon and Simpson indexes, P < 0.015).
- Bacterial community structure differed significantly between TSCC and normal tissues (Adonis and ANOSIM, P < 0.001).
- Specific taxa, including Neisseria, Streptococcus, and Actinomyces, were significantly reduced in cancer tissues, with a more conserved microbial community.
Conclusions:
- The oral microbial community is significantly altered in TSCC patients, with increased diversity and distinct compositional changes.
- These microbial alterations may play a role in the carcinogenic mechanisms of TSCC.
- Further research is warranted to elucidate the precise mechanisms by which microbial changes contribute to TSCC progression.
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