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Target-binding behavior of IDPs via pre-structured motifs
Do-Hyoung Kim1, Kyou-Hoon Han1
1Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
Progress in Molecular Biology and Translational Science
|October 17, 2021
Summary
Pre-Structured Motifs (PreSMos) are transient protein structures in intrinsically disordered proteins (IDPs). These PreSMos act as binding sites, enabling IDPs to interact with targets through conformational selection and induced fit mechanisms.
Area of Science:
- Biochemistry
- Structural Biology
- Protein Science
Background:
- Intrinsically disordered proteins (IDPs) lack stable tertiary structures.
- Understanding IDP function is challenging due to their dynamic nature.
- Pre-Structured Motifs (PreSMos) are transient secondary structures in IDPs.
Purpose of the Study:
- To define PreSMos as the functional 'active sites' of IDPs.
- To explain how PreSMos mediate target binding in IDPs.
- To provide a structural rationale for the function of unstructured proteins.
Main Methods:
- Analysis of PreSMo conformations in target-unbound states.
- Investigating the role of PreSMos in protein-target interactions.
- Discussing the sequential pathway of conformational selection (CS) and induced fit (IF).
Main Results:
- PreSMos exist a priori in IDPs, analogous to active sites in globular proteins.
- IDPs utilize PreSMos for target binding, not spatial pockets.
- PreSMos presage target-bound conformations, facilitating recognition via CS and IF.
Conclusions:
- PreSMos offer an atomic-resolution view of IDP structure and function.
- PreSMos are crucial for target recognition and binding in IDPs.
- This mechanism explains how IDPs can be both disordered and functional in disease contexts.
Keywords:
Completely unstructuredConformational selectionInduced fitMostly unstructuredPre-structured motifMore Related Videos
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