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Related Experiment Video

Updated: Oct 16, 2025

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Published on: August 13, 2021

Optimization of cell permeabilization in electron flow based mitochondrial function assays.

Xiang-He Lei¹, Barry R Bochner¹

¹Biolog, Inc., 21124 Cabot Blvd., Hayward, CA, 94545, USA.

Free Radical Biology & Medicine

|October 17, 2021

Summary

This summary is machine-generated.

Optimizing cell permeabilization is key for mitochondrial research. This study evaluates agents like saponin and digitonin using a novel redox dye assay for improved mitochondrial function studies.

Keywords:

Cell permeabilization Mitochondrial assay Mitochondrial function Mitochondrial inhibitors Mitochondrial metabolism Mitochondrial substrates

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Last Updated: Oct 16, 2025

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

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Published on: August 13, 2021

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Published on: October 26, 2021

Area of Science:

Biochemistry
Cell Biology
Mitochondrial Research

Background:

Permeable cell models are crucial for advancing mitochondrial research.
Effective permeabilization requires selective membrane permeability while preserving organelle integrity and function.

Purpose of the Study:

To assess and optimize commonly used permeabilizing agents for cell-based mitochondrial assays.
To evaluate the efficacy of saponin, digitonin, and perfringolysin O in maintaining mitochondrial function.

Main Methods:

Utilized a novel electron flow-based mitochondrial assay.
Employed a colorimetric redox dye for quantitative measurements.
Assessed various permeabilizing agents including saponin, digitonin, and recombinant perfringolysin O.

Main Results:

The study provides a comparative analysis of different permeabilizing agents.
Optimized conditions were determined for using permeabilized cells in mitochondrial assays.
The novel redox dye assay demonstrated effectiveness in evaluating mitochondrial function.

Conclusions:

This research offers guidance for optimizing cell permeabilization techniques in mitochondrial studies.
The findings support the use of the novel redox dye assay for reliable mitochondrial function assessment.
Proper agent selection and optimization are critical for successful permeable cell model applications.