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Influence of plasma components on luminol-enhanced chemiluminescence from peripheral granulocytes in juvenile
Abstract:
The generation rate of free oxygen radicals as measured by maximal light intensity of luminol-enhanced chemiluminescence from peripheral blood granulocytes (PMN) stimulated with differently opsonized Staphylococcus aureus was studied in 13 patients with juvenile periodontitis (JP) and pair-matched, healthy controls. Plasma proteins related to inflammation were also assayed. When stimulated with bacteria opsonized with autologous serum, the PMN from the JP patients showed a more intensive chemiluminescence than did their pair-matched controls (p less than or equal to 0.0005). The difference was consistent but slightly reduced when using heat-treated serum (p less than or equal to 0.006) or heterologous gammaglobulin (p less than or equal to 0.19) for opsonization. When testing freeze preserved sera from 11 of the compared pairs, the sera from JP patients induced a slightly higher chemiluminescence in PMN from a healthy donor (p less than or equal to 0.031). Protein analysis of the patient sera revealed a slightly higher concentration of complement 4 (p less than or equal to 0.032) and IgM (p less than or equal to 0.030) when compared with their respective pair-matched healthy controls. The influence of other blood components contaminating our assay system was checked on healthy PMN cells. Lymphocytes, platelets, relevant amounts of ADP and serum had no effect on the chemiluminescence. In conclusion, the increased chemiluminescence of peripheral blood granulocytes from patients with juvenile periodontitis seems to be related mainly to the cells. The association with free oxygen radicals and their tissue-damaging potency might be a contributing factor in the pathogenesis of periodontal disease.
Insights
Patients with juvenile periodontitis exhibit heightened free oxygen radical generation from their granulocytes when fighting Staphylococcus aureus. This increased cellular activity may contribute to the tissue damage seen in periodontal disease.
Area of Science:
- Immunology
- Periodontology
- Biochemistry
Background:
- Juvenile periodontitis (JP) is a severe gum disease.
- Free oxygen radical generation by immune cells plays a role in inflammation and tissue damage.
Purpose of the Study:
- To investigate the free oxygen radical generation rate in peripheral blood granulocytes (PMN) of patients with juvenile periodontitis (JP).
- To compare the chemiluminescence response of PMN from JP patients and healthy controls when stimulated with Staphylococcus aureus.
Main Methods:
- Luminol-enhanced chemiluminescence was used to measure free oxygen radical generation.
- Peripheral blood granulocytes (PMN) from 13 JP patients and matched controls were stimulated with opsonized Staphylococcus aureus.
- Plasma proteins, including complement 4 and IgM, were assayed.
Main Results:
- PMN from JP patients showed significantly higher chemiluminescence than controls when stimulated with autologous serum-opsonized bacteria (p ≤ 0.0005).
- This difference persisted with heat-treated serum (p ≤ 0.006) and heterologous gammaglobulin (p ≤ 0.19).
- JP patient sera induced higher chemiluminescence in healthy PMN (p ≤ 0.031), and JP patients had higher levels of complement 4 and IgM.
Conclusions:
- Increased chemiluminescence in JP patients' granulocytes is primarily cell-related.
- Elevated free oxygen radical production by PMN may contribute to the pathogenesis of juvenile periodontitis.