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Related Experiment Video

Updated: Oct 16, 2025

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Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers.

Ruoyu Zhong1, Shujie Yang1, Giovanni Stefano Ugolini2

  • 1Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|October 18, 2021
PubMed
Summary

A new acoustofluidic chip using a single-phase focused transducer (SPFT) offers efficient, biocompatible droplet sorting for drug screening. This technology achieves high throughput and purity at low power, improving single-cell analysis in drug development.

Keywords:
acoustofluidicsdroplet sortinghigh throughput sortingsingle phase focused transducer

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Droplet microfluidics enables single-cell drug screening but faces limitations in current devices.
  • Existing microfluidic sorters often require high voltages, exhibit low biocompatibility, and have limited throughput.

Purpose of the Study:

  • To introduce a novel single-phase focused transducer (SPFT)-based acoustofluidic chip for droplet sorting.
  • To demonstrate the superior performance of the SPFT sorter compared to existing microfluidic devices.

Main Methods:

  • Development of an SPFT-based acoustofluidic chip.
  • Characterization of the sorter's energy efficiency, accuracy, biocompatibility, throughput, and purity.
  • Application of the SPFT sorter for screening doxorubicin cytotoxicity on various cell types.

Main Results:

  • The SPFT sorter operates at low input power (<20 Vpp) with high energy transmission efficiency.
  • Achieved post-sorting cell viability of 93.5% and sorting purity up to 99.2%.
  • Demonstrated high throughput (>1000 events/second) and successful drug screening of doxorubicin cytotoxicity.

Conclusions:

  • The SPFT acoustofluidic chip offers a significant advancement over existing microfluidic droplet sorters.
  • This technology provides a fast, precise, and biocompatible platform for single-cell drug screening.
  • The SPFT sorter holds great potential for revolutionizing drug development and biomedical applications.