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Kerstin Thurow1, Heidi Fleischer2, Anna Bach2

  • 1Center for Life Science Automation CELISCA, Universität Rostock, F.-Barnewitz-Straße 8, D-18119 Rostock, Deutschland.

Biospektrum : Zeitschrift Der Gesellschaft Fur Biologishe Chemie (GBCH) Und Der Vereinigung Fur Allgemeine Und Angewandte Mikrobiologie (VAAM)
|October 18, 2021
PubMed
Summary

Automated testing reliably detects vitamin D2 and D3 levels in serum. This advancement is crucial for identifying vitamin deficiencies and preventing adverse health effects.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Nutritional Science

Context:

  • Vitamins are essential for numerous human physiological processes.
  • Identifying vitamin deficiencies is critical for maintaining health and preventing disease.
  • Automated analytical methods are necessary to meet the increasing demand for diagnostic testing.

Purpose:

  • To automate and compare the performance of three distinct methods for vitamin D determination in serum.
  • To evaluate the reliability and efficiency of automated vitamin D assays.

Summary:

  • Three methods for quantifying 25-hydroxyvitamin D2 (25(OH)D2) and 25-hydroxyvitamin D3 (25(OH)D3) in human serum were successfully automated.
  • Performance evaluation confirmed that all three automated methods provide reliable detection of both vitamin D2 and D3 metabolites.

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  • These methods are capable of quantifying vitamin D metabolites in the clinically relevant nanogram per milliliter (ng/ml) range.

    • Impact:

    • Enables widespread and efficient screening for vitamin D deficiency.
    • Supports early diagnosis and intervention, potentially mitigating associated health risks.
    • Facilitates large-scale clinical studies on vitamin D status and its health implications.