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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Oct 16, 2025

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

An Advanced Automated Patch Clamp Protocol Design to Investigate Drug-Ion Channel Binding Dynamics.

Peter Lukacs¹, Krisztina Pesti^2,3, Mátyás C Földi^1,2

¹Plant Protection Institute, Centre for Agricultural Research, Martonvásár, Hungary.

Frontiers in Pharmacology

|October 18, 2021

Summary

This study introduces a faster method using automated patch-clamp to analyze how sodium channel inhibitors work, revealing complex drug interactions for treating neurological disorders.

Keywords:

automated patch-clamp binding kinetics epilepsy lidocaine neuromuscular disorders pain riluzole sodium channel inhibitor

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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

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Last Updated: Oct 16, 2025

One-channel Cell-attached Patch-clamp Recording

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Published on: June 9, 2014

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Area of Science:

Pharmacology
Neuroscience
Biophysics

Background:

High-throughput screening often misses crucial drug mechanism details like binding kinetics.
Understanding drug-target interactions is vital for developing effective therapeutics.

Purpose of the Study:

To demonstrate that detailed mechanistic analysis is achievable with high throughput using automated patch-clamp.
To develop a rapid method for assessing sodium channel inhibitor mechanisms of action.

Main Methods:

Utilized the IonFlux Mercury microfluidics-based automated patch clamp.
Developed a complex voltage protocol repeated at 1 Hz for rapid kinetic analysis.
Monitored drug onset and offset kinetics during perfusion and washout.

Main Results:

Successfully assessed state-dependent association and dissociation kinetics of sodium channel inhibitors.
Observed complex, multi-step drug effects occurring on millisecond and second timescales.
Identified distinct sub-processes in drug-target interactions.

Conclusions:

The developed method enables rapid, in-depth mechanistic analysis of ion channel modulators.
This approach aids in evaluating the therapeutic potential of compounds for hyperexcitability disorders.
Detailed kinetic data can guide drug discovery for conditions like epilepsy and pain.