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YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by

Yan Wang1, Jinmei Xu1, Zhifeng Cheng1

  • 1Department of Endocrinology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

Experimental and Therapeutic Medicine
|October 18, 2021
PubMed

Insights

Yes-associated protein 1 (YAP1) promotes diabetic nephropathy by increasing inflammation and extracellular matrix deposition in kidney cells. YAP1 activates the NF-κB/JMJD3 pathway, contributing to diabetic nephropathy progression.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Endocrinology

Background:

  • Diabetic nephropathy (DN) is a severe complication of diabetes, characterized by glomerular mesangial cell (GMC) proliferation and extracellular matrix (ECM) deposition.
  • The role of Yes-associated protein 1 (YAP1) in the pathogenesis of DN is not well understood.

Purpose of the Study:

  • To investigate the regulatory effect and mechanism of YAP1 in high-glucose-induced inflammation and ECM deposition in GMCs.

Main Methods:

  • Established high-glucose-treated HBZY-1 cell models.
  • Assessed the impact of YAP1 on cell proliferation, inflammation, ECM deposition, and fibrosis.
  • Investigated the involvement of the NF-κB/Jumonji domain-containing protein D3 (JMJD3) signaling pathway.

Main Results:

  • YAP1 expression was elevated in high-glucose-treated HBZY-1 cells.
  • YAP1 silencing reduced cell viability, inflammatory cytokine levels, ECM deposition, and fibrosis.
  • Inhibitors of the NF-κB/JMJD3 pathway attenuated YAP1's pro-inflammatory and pro-fibrotic effects.

Conclusions:

  • YAP1 promotes high glucose-induced inflammation and ECM deposition in GMCs.
  • This promotion occurs via the activation of the NF-κB/JMJD3 signaling pathway.
Keywords:
NF-κB/JMJD3YAP1diabetic nephropathyextracellular matrix depositionglomerular mesangial cellsinflammation

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