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Updated: Oct 16, 2025

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Published on: May 10, 2016
Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
Jana Verbančič1,2, Jenny Jiahui Huang3, Heather E McFarlane3
1School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia.
Abstract:
We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be suitable for imaging other samples. Additionally, we offer free, open-source solutions for image analysis and provide extensive troubleshooting suggestions. For complete information on the use and execution of this protocol, please refer to McFarlane et al., 2021.

