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Updated: Oct 16, 2025

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
General Tolerance of Galactosyltransferases toward UDP-galactosamine Expands Their Synthetic Capability
Xuan Fu1,2, Madhusudhan Reddy Gadi1, Shuaishuai Wang1
1Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA.
Abstract:
Accessing large numbers of structurally diverse glycans and derivatives is essential to functional glycomics. We showed a general tolerance of galactosyltransferases toward uridine-diphosphate-galactosamine (UDP-GalN), which is not a commonly used sugar nucleotide donor. The property was harnessed to develop a two-step chemoenzymatic strategy for facile synthesis of novel and divergent N-acetylgalactosamine (GalNAc)-glycosides and derivatives in preparative scales. The discovery and the application of the new property of existing glycosyltransferases expand their catalytic capabilities in generating novel carbohydrate linkages, thus prompting the synthesis of diverse glycans and glycoconjugates for biological studies.
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