Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Propagation of Uncertainty from Systematic Error01:10

Propagation of Uncertainty from Systematic Error

1.0K
The atomic mass of an element varies due to the relative ratio of its isotopes. A sample's relative proportion of oxygen isotopes influences its average atomic mass. For instance, if we were to measure the atomic mass of oxygen from a sample, the mass would be a weighted average of the isotopic masses of oxygen in that sample. Since a single sample is not likely to perfectly reflect the true atomic mass of oxygen for all the molecules of oxygen on Earth, the mass we obtain from this...
1.0K
Traveling Waves: Lossless Lines01:27

Traveling Waves: Lossless Lines

217
The provided content explores the behavior of traveling waves on single-phase lossless transmission lines. It begins with a single-phase two-wire lossless transmission line of length Δx, characterized by a loop inductance LH/m and a line-to-line capacitance C F/m. These parameters result in a series inductance LΔx  and a shunt capacitance CΔx.
217
Propagation of Uncertainty from Random Error00:59

Propagation of Uncertainty from Random Error

1.3K
An experiment often consists of more than a single step. In this case, measurements at each step give rise to uncertainty. Because the measurements occur in successive steps, the uncertainty in one step necessarily contributes to that in the subsequent step. As we perform statistical analysis on these types of experiments, we must learn to account for the propagation of uncertainty from one step to the next. The propagation of uncertainty depends on the type of arithmetic operation performed on...
1.3K
Propagation Speed of Electromagnetic Waves01:30

Propagation Speed of Electromagnetic Waves

4.1K
Electromagnetic waves are consistent with Ampere's law. Assuming there is no conduction current Ampere's law is given as:
4.1K
Instrument Calibration01:12

Instrument Calibration

335
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
335
Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

48.4K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
48.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response Factor Correction for Quantitative Determination of Homooligomeric <i>Sso</i>SSB Binding to ssDNA by Native Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS.

Analytical chemistry·2026
Same author

Designing a Comparative Proteomics Experiment: Retention-Time Alignment and Imputation Algorithms Affect Statistical Comparisons between Samples.

Journal of proteome research·2025
Same author

Outer membrane vesicles from <i>Bacteroides fragilis</i> contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells.

bioRxiv : the preprint server for biology·2025
Same author

Molecular Dynamics Simulations of Native Protein Charging in Electrosprayed Droplets with Experimentally Relevant Compositions.

Journal of the American Chemical Society·2025
Same author

Electron Transfer Higher-Energy Collisional Dissociation Can Distinguish Cobalt-Adducted Isomers of Human Milk Oligosaccharides.

Analytical chemistry·2025

Related Experiment Video

Updated: Oct 16, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.5K

Propagating Error through Traveling-Wave Ion Mobility Calibration.

Alexis N Edwards1, Hien M Tran1, Elyssia S Gallagher1

  • 1Department of Chemistry and Biochemistry, Baylor University, One Bear Place #97348, Waco, Texas 76798, United States.

Journal of the American Society for Mass Spectrometry
|October 18, 2021
PubMed
Summary

Native ion mobility spectrometry (IM-MS) provides insights into protein structures. Properly accounting for calibration uncertainty in traveling-wave IM-MS (TWIMS) improves structural comparisons with gas-phase data.

More Related Videos

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity
11:30

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity

Published on: March 6, 2017

11.9K
Calibration of Vector Network Analyzer for Measurements in Radio Frequency Propagation Channels
10:00

Calibration of Vector Network Analyzer for Measurements in Radio Frequency Propagation Channels

Published on: June 2, 2020

21.6K

Related Experiment Videos

Last Updated: Oct 16, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.5K
Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity
11:30

Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity

Published on: March 6, 2017

11.9K
Calibration of Vector Network Analyzer for Measurements in Radio Frequency Propagation Channels
10:00

Calibration of Vector Network Analyzer for Measurements in Radio Frequency Propagation Channels

Published on: June 2, 2020

21.6K

Area of Science:

  • Biophysical Chemistry
  • Structural Biology
  • Mass Spectrometry

Background:

  • Native mass spectrometry (MS) analyzes protein complexes in the gas phase, but ionization alters structures from solution.
  • Gas-phase protein conformations offer insights into solution-phase energy landscapes.
  • Ion mobility (IM) spectrometry characterizes gas-phase protein structures.

Purpose of the Study:

  • To investigate gas-phase protein conformations using ion mobility spectrometry.
  • To assess the impact of calibration uncertainty on traveling-wave ion mobility spectrometry (TWIMS) measurements.
  • To improve the comparability of gas-phase protein structures derived from TWIMS with other experimental and computational data.

Main Methods:

  • Calibrated a traveling-wave ion mobility spectrometry (TWIMS) instrument.
  • Derived collision cross-section (CCS) values (TWCCS$_{N2}$ and TWCCS$_{N2→He}$) for four proteins.
  • Propagated calibration error through TWIMS measurements.

Main Results:

  • Reporting TWIMS collision cross-section (CCS) values with propagated calibration uncertainty significantly increased agreement with literature drift tube CCS (DT-CCS) values.
  • This approach provides a more comprehensive assessment of protein ion gas-phase conformations.
  • Four proteins (cytochrome c, ubiquitin, apo-myoglobin, holo-myoglobin) were analyzed.

Conclusions:

  • Accurate error propagation in TWIMS calibration is crucial for reliable structural analysis.
  • Improved gas-phase structural data enhances comparisons between native IM-MS and other structural datasets.
  • This method refines the understanding of protein conformational landscapes in the gas phase.