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Analysis of Herba Asari polysaccharides and their immunological activity
Jingjing Li1, Li Ji2, Lin Sun2
1Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China; College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Abstract:
A water-soluble polysaccharide (HA) was extracted from the Herba Asari root. HA was separated into a starch-like glucan fraction (HA1) and a pectin fraction (HA2) using DEAE-cellulose. HA2 was further fractionated into three pectic polysaccharides, HA2-a, HA2-b and HA2-c, using ion-exchange chromatography. NMR and sugar composition analyses demonstrated that HA2-a is an arabinogalactan (AG) and HA2-b and HA2-c are xylogalacturonans (XGA) with AG domains. Lymphocyte proliferation assays showed that both the neutral polysaccharide and acidic polysaccharide were potent B and T cell stimulators that may have two different modes of action.
