Related Experiment Video
Updated: Oct 16, 2025
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
In vitro prebiotic evaluation of exopolysaccharides produced by marine isolated lactic acid bacteria
Tipparat Hongpattarakere1, Nantina Cherntong1, Santad Wichienchot2
1Department of Industrial Biotechnology, Faculty of Agro-industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Abstract:
Lactic acid bacteria (LAB) selected based on high EPSs production yields of 14, 7.6, 4.9 and 5g/L in sucrose containing MRS broth were identified as Weissella cibaria, Weissella confusa, Lactobacillus plantarum and Pediococcus pentosaceus, respectively based on their 16S rDNA sequences. EPSs produced by these strains did not stimulate secretion of interleukin (IL)-8, and were resistant to stomach acid and human pancreatic amylase. In pure culture system, only Bifidobacterium bifidum DSM 20456 exhibited the ability to utilize these EPSs as carbon sources but not L. plantarum TISTR 875 and Lactobacillus acidophilus TISTR 1034. EPSs from W. cibaria exhibited strong bifidogenic effect in the mixed-culture of human fecal microflora using the three-stage fermentation model. In the transverse and distal colon, bifidobacteria and lactobacilli as well as acetate and propionate increased significantly. Butyrate slightly decreased in the proximal colon region after feeding EPSs, but increased in the distal region.
