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Genic microsatellite marker characterization and development in little millet (Panicum sumatrense) using
Hiral Desai1, Rasmieh Hamid2, Zahra Ghorbanzadeh3
1Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat, India.
Researchers identified 4,443 microsatellite markers in little millet (Setaria italica) transcriptomes. These simple sequence repeat (SSR) markers are valuable for improving climate-resilient crops through genomic breeding and conservation efforts.
Area of Science:
- Genomics
- Plant Science
- Molecular Biology
Background:
- Little millet is a climate-resilient crop with high nutritional value.
- Limited molecular markers hinder genomic approaches in millet breeding.
Purpose of the Study:
- To identify and characterize genic-SSR motifs in the little millet transcriptome.
- To develop molecular markers for enhancing millet breeding and genetic studies.
Main Methods:
- Transcriptome sequencing of three little millet samples.
- Identification and analysis of genic-SSR motifs in unigene sequences.
- Validation of developed SSR primers in various millet genotypes.
Main Results:
- 4,443 genic-SSR motifs were identified in 30,220 unigene sequences.
- Tri-nucleotide repeats were the most abundant type (66.67%).
- 48% of validated primers showed polymorphism with 70% transferability across genotypes.
Conclusions:
- The identified SSRs represent a valuable resource for little millet genetic research.
- These markers can support quantitative trait loci (QTL)-based breeding and genetic conservation.
- Microsatellites facilitate marker-assisted selection (MAS) and evolutionary genetics studies in millets.
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