Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.0K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo design of DNA origami with a generative diffusion model.

Nature communications·2026
Same author

Classifying Multistate DNA Origami: An Automated Approach with Minimal Labeling and Confidence-Based Filtering.

Journal of chemical information and modeling·2026
Same author

Harnessing Longitudinal Health Data for Aging Populations.

JAMA network open·2026
Same author

Quantitative and visual evaluation of dynamic isotropy in dual-axis random positioning machine (RPM) or clinostat for ground-based microgravity simulation.

Biomedical engineering letters·2026
Same author

Genome-wide association study of frailty and integrative functional analysis to elucidate its relationship with aging.

GeroScience·2026
Same author

Uncovering Design and Assembly Rules for mRNA-DNA Origami.

Nano letters·2026

Related Experiment Video

Updated: Oct 16, 2025

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.0K

The flexibility-based modulation of DNA nanostar phase separation.

Taehyun Lee1, Sungho Do1, Jae Gyung Lee1

  • 1Department of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea. ydshin@snu.ac.kr.

Nanoscale
|October 19, 2021
PubMed
Summary

Structural flexibility in DNA nanostars inhibits biomolecular phase separation. This finding reveals insights into cellular regulation and synthetic assembly control, impacting understanding of physiological compartmentalization.

More Related Videos

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

13.2K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.3K

Related Experiment Videos

Last Updated: Oct 16, 2025

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.0K
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

13.2K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.3K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Biomolecular phase separation is crucial for cellular functions and disease.
  • Understanding the link between molecular interactions and phase behavior is essential.
  • DNA nanostars offer a tunable platform to study these interactions.

Purpose of the Study:

  • To investigate the impact of structural flexibility on DNA nanostar phase separation.
  • To elucidate the relationship between intermolecular forces and phase behavior in synthetic systems.
  • To explore potential cellular regulatory mechanisms and synthetic assembly control.

Main Methods:

  • Design and synthesis of DNA nanostars with varying degrees of flexibility using single-stranded gaps.
  • Systematic study of phase behaviors of these DNA nanostars.
  • Thermodynamic analysis to understand the underlying mechanisms.

Main Results:

  • Increased structural flexibility significantly inhibits the phase separation of DNA nanostars.
  • This inhibition is attributed to a generic, flexibility-driven thermodynamic change, not valency loss.
  • Phase diagrams are drastically altered by varying nanostar flexibility.

Conclusions:

  • Structural flexibility is a critical determinant of biomolecular phase separation.
  • Findings suggest potential cellular mechanisms for dynamically controlling intracellular phase separation.
  • Provides a framework for designing synthetic systems with signal-dependent assembly.