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Updated: Oct 16, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

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miR‑497/MIR497HG inhibits glioma cell proliferation by targeting CCNE1 and the miR‑588/TUSC1 axis

Li-Ya Ji1, Ming Wei2, Yuan-Yuan Liu2

  • 1Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710054, P.R. China.

Oncology Reports
|October 19, 2021
PubMed

Insights

MicroRNA-497 and its host gene MIR497HG are downregulated in glioma, acting as tumor suppressors. Low expression correlates with poor prognosis, suggesting their potential as therapeutic targets and biomarkers for glioma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-497 (miR-497) is implicated in various cancers, affecting tumorigenesis, progression, metastasis, and chemotherapy resistance.
  • The role and expression of the miR-497 host gene (MIR497HG) in glioma remain largely unexplored.

Purpose of the Study:

  • To investigate the expression patterns and biological functions of miR-497 and MIR497HG in glioma.
  • To determine the association between miR-497/MIR497HG expression and patient prognosis.
  • To identify downstream targets and elucidate the underlying mechanisms of miR-497/MIR497HG in glioma.

Main Methods:

  • Quantitative analysis of miR-497 and MIR497HG expression in glioma tissues and cell lines.
  • Bioinformatics analysis, luciferase reporter assays, and functional experiments (e.g., cell proliferation, cell cycle assays).
  • Correlation analysis between gene expression levels and patient survival data.

Main Results:

  • miR-497 and MIR497HG were significantly downregulated in glioma tissues and cell lines compared to normal controls.
  • Lower expression levels of miR-497 or MIR497HG were associated with poorer prognostic outcomes in glioma patients.
  • Overexpression of miR-497 or MIR497HG suppressed glioma cell proliferation and cell cycle progression.
  • miR-497 and MIR497HG directly target cyclin E1 and miR-588/tumor suppressor candidate 1.

Conclusions:

  • miR-497 and MIR497HG function as tumor suppressors in glioma.
  • These molecules represent potential therapeutic targets and prognostic biomarkers for glioma management.
Keywords:
MIR497HGcyclin E1gliomamiR‑497miR‑588tumor suppressor candidate 1

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