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Updated: Oct 16, 2025
Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
Published on: June 13, 2014
High-sensitive photometric microplate assay for tumor necrosis factor-alpha based on Fe@BC nanozyme
Xubiao Xie1, Liang Tan1, Siyuan Liu2
1Department of Organ Transplantation Center, The Second Xiang-ya Hospital, Central South University, Changsha 410011, China; Transplant Medical Research Center, The Second Xiang-ya Hospital, Central South University, Changsha 410011, China.
Abstract:
Optical immunoassay for the detection of protein biomarker tumor necrosis factor-α (TNF-α) is reported based on core-shell Fe@BC structure nanozyme as label. The nanozyme is composed of Fe nanoparticles as core and carbon layer as shell with element B doped into the carbon shell. The nanozyme displays good peroxidase-like activity that can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to oxTMB in the presence of H2O2, generating blue colored compound. The detection of TNF-α was following the ELISA protocol by replacing traditional enzyme horseradish peroxidase (HRP) with nanozyme Fe@BC. The assay can be finished by about 2 h and has wide linear range for TNF-α from 5 pg/mL to 1 ng/mL with limit of detection of 2 pg/mL. The assay was applied for the detection of TNF-α in human serum samples with detection results comparable to commercial ELISA.
