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TM7SF2 regulates cell proliferation and apoptosis by activation of C-Raf/ERK pathway in cervical cancer

Yichi Xu1, Xin Chen1, Shuya Pan1

  • 1Center for Uterine Cancer Diagnosis & Therapy Research of Zhejiang Province, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.

Cell Death Discovery
|October 20, 2021
PubMed

Insights

Transmembrane 7 superfamily member 2 (TM7SF2) promotes cervical cancer growth and metastasis by activating the C-Raf/ERK pathway. Targeting TM7SF2 may offer a new therapeutic strategy for cervical cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Transmembrane 7 superfamily member 2 (TM7SF2) is involved in cholesterol metabolism and its role in cancer progression is largely unknown.
  • Understanding TM7SF2's function in cervical cancer is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of TM7SF2 in the regulation of cervical cancer cell growth, proliferation, migration, invasion, and apoptosis.
  • To elucidate the underlying molecular mechanisms, including the involvement of the C-Raf/ERK pathway.

Main Methods:

  • Immunohistochemistry (IHC), real-time RT-PCR, and western blotting were used to assess TM7SF2 expression in cervical cancer tissues.
  • In vitro assays (CCK8, colony formation, Transwell, wound healing, flow cytometry) evaluated cellular functions after TM7SF2 modulation.
  • In vivo xenograft models assessed tumor growth.

Main Results:

  • Upregulation of TM7SF2 enhanced cervical cancer cell proliferation, migration, and invasion while suppressing apoptosis.
  • TM7SF2 knockout reversed these effects, indicating its oncogenic role.
  • TM7SF2 overexpression promoted xenograft tumor growth and activated the C-Raf/ERK pathway.

Conclusions:

  • TM7SF2 plays a significant role in promoting cervical cancer tumorigenesis and progression through C-Raf/ERK pathway activation.
  • TM7SF2 represents a potential therapeutic target for cervical cancer treatment.

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