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Related Experiment Video

Updated: Oct 16, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Published on: December 16, 2016

Proposal for an initial screening method for identifying microplastics in marine sediments.

Toshiro Hata¹, Ningjun Jiang²

¹Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8527, Japan. thata@hiroshima-u.ac.jp.

Scientific Reports

|October 20, 2021

Summary

A new, safe, and easy protocol effectively identifies microplastics in marine sediments. This method uses standard geotechnical equipment for enhanced microplastic analysis in sediment fractions.

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Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

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Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris

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Separation and Identification of Conventional Microplastics from Farmland Soils

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Area of Science:

Environmental Science
Geology
Marine Biology

Background:

Marine debris, specifically microplastics, are prevalent environmental hazards in marine ecosystems.
Microplastics in sediments pose risks through contaminant concentration, biofilm formation, and ingestion by benthic organisms, impacting higher food chains.

Purpose of the Study:

To develop and validate a novel, safe, and efficient protocol for identifying microplastics within various marine sediment fractions.

Main Methods:

The protocol integrates sieving, adapted from particle size distribution tests, with nontoxic dye staining.
It utilizes conventional geological and geotechnical testing equipment, ensuring accessibility and ease of use.

Main Results:

The developed protocol successfully stained and categorized diverse microplastic types and sizes from contaminated sediment samples.
The method proved effective in analyzing microplastics across different sediment fractions.

Conclusions:

This innovative protocol offers a safe, user-friendly, and efficient alternative for studying microplastics in marine sediments.
It provides a foundation for geotechnical and geological engineers to analyze microplastics using familiar materials and methods.