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Updated: Oct 16, 2025
A Mouse Model for Laser-induced Choroidal Neovascularization
Published on: December 27, 2015
Therapeutic effect of a traditional Chinese medicine formulation on experimental choroidal neovascularization in
Yu-Fei Zhang1,2, Chuan Jiang1,3, Xiao-Hong Zhou4
1Center of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Aim:
To investigate therapeutic effects of traditional Chinese medicine formulations, Hexuemingmu (HXMM) on laser-induced choroidal neovascularization (CNV) and follow-up effect in mice.
Methods:
C57BL/6 mice of 8-week-old were used and CNV was induced with 577 nm laser photocoagulation. Animals were randomly divided into groups and different doses of HXMM were administered daily. One, four, and eight weeks after the intervention, the electroretinogram (ERG), fundus fluorescence angiography, choroidal flat mount and immunofluorescence staining were preformed to evaluate the function and CNV formation. The expression levels of angiogenic proteins were determined by Western blotting and immunofluorescence staining. An analysis of variance and Kruskal-Wallis test were used to test the differences among the groups.
Results:
The results showed that HXMM effectively increased amplitude of ERG of mice (P<0.05), alleviated fundus CNV leakage (P<0.05), and reduced the area of neovascularization and the expression of angiogenic proteins (P<0.05) after laser-induced CNV.
Conclusion:
HXMM can protect the retinal function of mice after laser-induced CNV, and inhibit the CNV development.
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