A plant reovirus hijacks the DNAJB12-Hsc70 chaperone complex to promote viral spread in its planthopper vector

Many viruses usurp the functions of endoplasmic reticulum (ER) for virus-encoded membrane proteins proper functional folding or assembly to promote virus spread. Southern rice black-streaked dwarf virus (SRBSDV), a plant reovirus, exploits virus-containing tubules composed of nonstructural membrane protein P7-1 to spread in its planthopper vector Sogatella furcifera. Here, we report that two factors of the ER-associated degradation (ERAD) machinery, the ER chaperone DNAJB12 and its cytosolic co-chaperone Hsc70, are activated by SRBSDV to facilitate ER-to-cytosol export of P7-1 tubules in S. furcifera. Both P7-1 of SRBSDV and Hsc70 directly bind to the J-domain of DNAJB12. DNAJB12 overexpression induces ER retention of P7-1, but Hsc70 overexpression promotes the transport of P7-1 from the ER to the cytosol to initiate tubule assembly. Thus, P7-1 is initially retained in the ER by interaction with DNAJB12 and then delivered to Hsc70. Furthermore, the inhibitors of the ATPase activity of Hsc70 reduce P7-1 tubule assembly, suggesting that the proper folding and assembly of P7-1 tubules is dependent on the ATPase activity of Hsc70. The DNAJB12-Hsc70 chaperone complex is recruited to P7-1 tubules in virus-infected midgut epithelial cells in S. furcifera. The knockdown of DNAJB12 or Hsc70 strongly inhibits P7-1 tubule assembly in vivo, finally suppressing effective viral spread in S. furcifera. Taken together, our results indicate that the DNAJB12-Hsc70 chaperone complex in the ERAD machinery facilitates the ER-to-cytosol transport of P7-1 for proper assembly of tubules, enabling viral spread in insect vectors in a manner dependent on ATPase activity of Hsc70.